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A) Single-mode illumination path(s) (blue): The single-mode laser(s) are spatially filtered, expanded and homogenized. The resulting flat-top intensity profile is further expanded by exchangeable telescopes to control the size of the illumination field. Setting the angle for TIRF/HILO illumination is accomplished by positioning of a motorized mirror. The laser power is monitored in real time by measuring the <1% transmission of the laser lines at the illumination dichroic mirror. Emission path (green): A primary image is produced by a tube lens, restricted in spatial extent by a slit and relayed in a 4f <t>configuration</t> via a spectrally discriminative image splitter to form two secondary images on an <t>sCMOS</t> camera. A variable astigmatic lens (cylindrical lens pair) can be removed/inserted for 2D/3D imaging, while a Bertrand lens allows viewing of the objective back focal plane. Focus lock path (red): The focus lock laser is separated from emitted fluorescence by the emission dichroic mirror, focused to the objective back focal plane and launched obliquely to return under the TIRF condition allowing axial drift of the coverslip relative to the objective lens to be measured via a quadrant photodiode and corrected via the control loop of the piezo flexure stage mounting the objective. B) For clarity the multi-mode laser illumination path (orange) has been shown separately but all modules are co-compatible. A magnified image of the tip of a square core multimode fiber (coupled to a multi-mode laser engine) is produced and re-imaged onto the sample via a tube lens in a retrofocus arrangement and the objective lens. The multi-mode laser is coupled into the microscope by translation of the motorized mirror (HILO/TIRF) fully out of the beam path
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A) Single-mode illumination path(s) (blue): The single-mode laser(s) are spatially filtered, expanded and homogenized. The resulting flat-top intensity profile is further expanded by exchangeable telescopes to control the size of the illumination field. Setting the angle for TIRF/HILO illumination is accomplished by positioning of a motorized mirror. The laser power is monitored in real time by measuring the <1% transmission of the laser lines at the illumination dichroic mirror. Emission path (green): A primary image is produced by a tube lens, restricted in spatial extent by a slit and relayed in a 4f <t>configuration</t> via a spectrally discriminative image splitter to form two secondary images on an <t>sCMOS</t> camera. A variable astigmatic lens (cylindrical lens pair) can be removed/inserted for 2D/3D imaging, while a Bertrand lens allows viewing of the objective back focal plane. Focus lock path (red): The focus lock laser is separated from emitted fluorescence by the emission dichroic mirror, focused to the objective back focal plane and launched obliquely to return under the TIRF condition allowing axial drift of the coverslip relative to the objective lens to be measured via a quadrant photodiode and corrected via the control loop of the piezo flexure stage mounting the objective. B) For clarity the multi-mode laser illumination path (orange) has been shown separately but all modules are co-compatible. A magnified image of the tip of a square core multimode fiber (coupled to a multi-mode laser engine) is produced and re-imaged onto the sample via a tube lens in a retrofocus arrangement and the objective lens. The multi-mode laser is coupled into the microscope by translation of the motorized mirror (HILO/TIRF) fully out of the beam path
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A) Single-mode illumination path(s) (blue): The single-mode laser(s) are spatially filtered, expanded and homogenized. The resulting flat-top intensity profile is further expanded by exchangeable telescopes to control the size of the illumination field. Setting the angle for TIRF/HILO illumination is accomplished by positioning of a motorized mirror. The laser power is monitored in real time by measuring the <1% transmission of the laser lines at the illumination dichroic mirror. Emission path (green): A primary image is produced by a tube lens, restricted in spatial extent by a slit and relayed in a 4f <t>configuration</t> via a spectrally discriminative image splitter to form two secondary images on an <t>sCMOS</t> camera. A variable astigmatic lens (cylindrical lens pair) can be removed/inserted for 2D/3D imaging, while a Bertrand lens allows viewing of the objective back focal plane. Focus lock path (red): The focus lock laser is separated from emitted fluorescence by the emission dichroic mirror, focused to the objective back focal plane and launched obliquely to return under the TIRF condition allowing axial drift of the coverslip relative to the objective lens to be measured via a quadrant photodiode and corrected via the control loop of the piezo flexure stage mounting the objective. B) For clarity the multi-mode laser illumination path (orange) has been shown separately but all modules are co-compatible. A magnified image of the tip of a square core multimode fiber (coupled to a multi-mode laser engine) is produced and re-imaged onto the sample via a tube lens in a retrofocus arrangement and the objective lens. The multi-mode laser is coupled into the microscope by translation of the motorized mirror (HILO/TIRF) fully out of the beam path
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line-scanning push-broom-configured camera vlnircl-100-n173  (Specim Spectral Imaging)
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Specim Spectral Imaging line-scanning push-broom-configured camera vlnircl-100-n173
A) Single-mode illumination path(s) (blue): The single-mode laser(s) are spatially filtered, expanded and homogenized. The resulting flat-top intensity profile is further expanded by exchangeable telescopes to control the size of the illumination field. Setting the angle for TIRF/HILO illumination is accomplished by positioning of a motorized mirror. The laser power is monitored in real time by measuring the <1% transmission of the laser lines at the illumination dichroic mirror. Emission path (green): A primary image is produced by a tube lens, restricted in spatial extent by a slit and relayed in a 4f <t>configuration</t> via a spectrally discriminative image splitter to form two secondary images on an <t>sCMOS</t> camera. A variable astigmatic lens (cylindrical lens pair) can be removed/inserted for 2D/3D imaging, while a Bertrand lens allows viewing of the objective back focal plane. Focus lock path (red): The focus lock laser is separated from emitted fluorescence by the emission dichroic mirror, focused to the objective back focal plane and launched obliquely to return under the TIRF condition allowing axial drift of the coverslip relative to the objective lens to be measured via a quadrant photodiode and corrected via the control loop of the piezo flexure stage mounting the objective. B) For clarity the multi-mode laser illumination path (orange) has been shown separately but all modules are co-compatible. A magnified image of the tip of a square core multimode fiber (coupled to a multi-mode laser engine) is produced and re-imaged onto the sample via a tube lens in a retrofocus arrangement and the objective lens. The multi-mode laser is coupled into the microscope by translation of the motorized mirror (HILO/TIRF) fully out of the beam path
Line Scanning Push Broom Configured Camera Vlnircl 100 N173, supplied by Specim Spectral Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/camera+configuration/pm34303815-134-8-10?v=Specim+Spectral+Imaging
Average 90 stars, based on 1 article reviews
line-scanning push-broom-configured camera vlnircl-100-n173 - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


A) Single-mode illumination path(s) (blue): The single-mode laser(s) are spatially filtered, expanded and homogenized. The resulting flat-top intensity profile is further expanded by exchangeable telescopes to control the size of the illumination field. Setting the angle for TIRF/HILO illumination is accomplished by positioning of a motorized mirror. The laser power is monitored in real time by measuring the <1% transmission of the laser lines at the illumination dichroic mirror. Emission path (green): A primary image is produced by a tube lens, restricted in spatial extent by a slit and relayed in a 4f configuration via a spectrally discriminative image splitter to form two secondary images on an sCMOS camera. A variable astigmatic lens (cylindrical lens pair) can be removed/inserted for 2D/3D imaging, while a Bertrand lens allows viewing of the objective back focal plane. Focus lock path (red): The focus lock laser is separated from emitted fluorescence by the emission dichroic mirror, focused to the objective back focal plane and launched obliquely to return under the TIRF condition allowing axial drift of the coverslip relative to the objective lens to be measured via a quadrant photodiode and corrected via the control loop of the piezo flexure stage mounting the objective. B) For clarity the multi-mode laser illumination path (orange) has been shown separately but all modules are co-compatible. A magnified image of the tip of a square core multimode fiber (coupled to a multi-mode laser engine) is produced and re-imaged onto the sample via a tube lens in a retrofocus arrangement and the objective lens. The multi-mode laser is coupled into the microscope by translation of the motorized mirror (HILO/TIRF) fully out of the beam path

Journal: bioRxiv

Article Title: Automated 3D multi-color single-molecule localization microscopy

doi: 10.1101/2023.10.23.563122

Figure Lengend Snippet: A) Single-mode illumination path(s) (blue): The single-mode laser(s) are spatially filtered, expanded and homogenized. The resulting flat-top intensity profile is further expanded by exchangeable telescopes to control the size of the illumination field. Setting the angle for TIRF/HILO illumination is accomplished by positioning of a motorized mirror. The laser power is monitored in real time by measuring the <1% transmission of the laser lines at the illumination dichroic mirror. Emission path (green): A primary image is produced by a tube lens, restricted in spatial extent by a slit and relayed in a 4f configuration via a spectrally discriminative image splitter to form two secondary images on an sCMOS camera. A variable astigmatic lens (cylindrical lens pair) can be removed/inserted for 2D/3D imaging, while a Bertrand lens allows viewing of the objective back focal plane. Focus lock path (red): The focus lock laser is separated from emitted fluorescence by the emission dichroic mirror, focused to the objective back focal plane and launched obliquely to return under the TIRF condition allowing axial drift of the coverslip relative to the objective lens to be measured via a quadrant photodiode and corrected via the control loop of the piezo flexure stage mounting the objective. B) For clarity the multi-mode laser illumination path (orange) has been shown separately but all modules are co-compatible. A magnified image of the tip of a square core multimode fiber (coupled to a multi-mode laser engine) is produced and re-imaged onto the sample via a tube lens in a retrofocus arrangement and the objective lens. The multi-mode laser is coupled into the microscope by translation of the motorized mirror (HILO/TIRF) fully out of the beam path

Article Snippet: The instructions provided assume the use of the base configuration Hamamatsu Fusion BT sCMOS camera.

Techniques: Control, Transmission Assay, Produced, Imaging, Fluorescence, Microscopy

Top: as viewed from the front/right of the microscope. From left-right: the tube lens, field aperture, first steering mirror, first relay lens, laser blocking filters, astigmatic lens and filter wheels either side of the image splitter dichroic mirror. Bottom: as viewed from the back/left of the microscope highlighting the image splitter platform. Following the filter wheel, the transmitted and reflected paths each comprise a second relay lens and steering mirror before the images are recombined by a knife-edge mirror (occluded in figure) and imaged onto an sCMOS camera.

Journal: bioRxiv

Article Title: Automated 3D multi-color single-molecule localization microscopy

doi: 10.1101/2023.10.23.563122

Figure Lengend Snippet: Top: as viewed from the front/right of the microscope. From left-right: the tube lens, field aperture, first steering mirror, first relay lens, laser blocking filters, astigmatic lens and filter wheels either side of the image splitter dichroic mirror. Bottom: as viewed from the back/left of the microscope highlighting the image splitter platform. Following the filter wheel, the transmitted and reflected paths each comprise a second relay lens and steering mirror before the images are recombined by a knife-edge mirror (occluded in figure) and imaged onto an sCMOS camera.

Article Snippet: The instructions provided assume the use of the base configuration Hamamatsu Fusion BT sCMOS camera.

Techniques: Microscopy, Blocking Assay